Vivaflow 200 reusable laboratory crossflow cassette From Sartorius Stedim Biotech

Vivaflow 200 reusable laboratory crossflow cassette From Sartorius Stedim Biotech

New, Unused, from a Lab Clearance, still in Manufacturers packaging. Half Price £145.00 Each

We also have Masterflex pumps with EasyLoad 2 and Easy Load LS Heads. Which are needed to set up the system.

Vivaflow 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaniously.

Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breading facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single rinse. Vivaflow 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.
Equipment required

The standard Vivaflow 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).
Running 2 Vivaflow 200 cassettes in parallel

For speeding up the concentration, two Vivaflow 200 cassettes can easily be connected using a Y connector which is preassembeled with the correct tubing sizes and fittings (size 16 tubing for connecting the two Vivaflow 200 units, size 15 tubing for the pump). Please note that you need a size 15 pump head for running 2 Vivaflows in parallel.

•     High flow rate and recovery
•  
•     Clear housing for process monitoring
•  
•     Ready to use single cassettes
•  
•     Easy set up of 2 cassettes
•  
•     Re-usable product

Performance Characteristics

      Time to concentrate up to 20x (min.) at 3 bar
inlet pressure, 20°C                 
      Start volume     Average flux     Recovery %     
      1 litre     ml/min     Direct     25 ml rinse
Lysozyme 0.25 mg/ml (14,000 MW)                       
2,000 MWCO Hydrosart     160     6     97 %     > 99 %
3,000 MWCO PES     180     5     97 %     > 99 %
BSA 1.0 mg/ml (66,000 MW)                       
5,000 MWCO PES     29     33     98 %     > 99 %
5,000 MWCO Hydrosart     70     14     98 %     > 99 %
10,000 MWCO PES     23     41     96 %     > 99 %
10,000 MWCO Hydrosart     35     27     98 %     > 99 %
30,000 MWCO PES     25     38     96 %     99 %
30,000 MWCO Hydrosart     20     48     96 %     > 99 %
50,000 MWCO PES     22     43     96 %     98 %
? Globulins 1.0 mg/ml (average 160,000 MW)                       
100,000 MWCO PES     54     18     96 %     99 %
100,000 MWCO RC     45     21     96 %     99 %
Yeast 1.0 mg/ml (S. Cerevisiae)                       
0.2 µm PES     11     86     92 %     98 %
Dilute solute concentration, start volume
1 litre at 3 bar, 10,000 MWCO PES                       
BSA 0.001 mg/ml     18     52     90 %     98 %
BSA 0.01 mg/ml     20     47     92 %     98 %
BSA 0.1 mg/ml     21     45     94 %     99 %
Start volume 5 litres (two VF200 in
parallel at 3 bar) 10,000 MWCO PES                       
BSA 1.0 mg/ml (66,000 MW)     67     70     97 %     > 99 %


 Product properties

Market    Research
Application    Concentration;Desalting | buffer exchange
Type of sample    Nanoparticles;Peptide;Protein
Ultrafiltration device type    Tangential | crossflow concentrator
Membrane    Polyethersulfone (PES)
MWCO    5.000 Dalton
Type    Tangential | crossflow concentrator
Volume Range    500 - 5.000 ml
Certification    None
Technical attributes

• Overall length    126 mm
• Overall height    138 mm
• Overall width    38 mm
• Channel width    10 mm
• Channel height    0,4 mm
• Active Membrane Area    200 cm²
• Hold-up volume    5,3 ml
• Minimum Recirculation Volume    < 20 ml
• Max. temperature    60 °C
• Non-recoverable Hold-up    < 2 ml
• Pump Flow    200 - 400 ml/min
• Operating pressure max.    4 bar
• Main Housing    Acrylic
• Flow Channel    Acrylic
• Membrane support    Polypropylene (PP)
• Seals and O-rings    Silicone
• Pressure indicator    Polypropylene, SS spring
• Flow Restrictor    Polypropylene (PP)
• Fittings    Nylon (NY)
• Tubing    PVC (medical grade)