TTP LabTech Mirrorball Fluorescence Cytometer

STP LabTech Mirrorball Fluorescence Cytometer HTS Antibody Screening Discovery

The mirrorball high sensitivity cytometer represents one of the most versatile instruments available for antibody screening. The system embodies SPTLabtech’s laser scanning technology and enables streamlined workflows for unprecedented levels of throughput.

Benefits:

• No wash steps required – Sample integrity is retained; antibody interactions with low affinity and/or high dissociation rates can be detected
• Retains antigen conformation – able to scan adherent cells without any manipulation
• Streamline workflow – combines assays for antibody binding, specificity and cell interaction required for primary antibody screening, into a single test tube
• Reduced antigen requirement – bead-based assays optimise antigen usage
• Greater throughput and sensitivity – combination of mix-and-read protocols, automation-friendly software and robotics allows mirroball to deliver simply unbeatable throughput and sensitivity (up to 150,000 samples a day)
• Multiplexing – triple laser excitation and 5 colour fluorescence detection enable analysis of multiple antigens or cell types simultaneously

Applications:

1. Screening tool for all stages of the antibody discovery process from primary screening, clone selection and affinity maturation
2. Protocols have been set up for the most common antibody species including whole IgG, scFV and Fab
3. Cell-based assays:

• • allows screening of antigens in their natural conformation
  • preserves target epitopes
  • improves productivity by delivering quality screening hits

Supplied with Cellista control and off-line analysis software V4.3.5. Recently taken from an upgraded antibody drug discovery robotic system in full working condition. Transported using manufactures travel lock. Images from part of the description.

Specification:

• Detection technology: Laser scanning fluorescence cytometry
• Laser excitation: Up to 3 lasers in a single instrument 405, 488, 640 nm
• Sample format: 96, 384 and 1536 SBS-format microplates
• Throughput: Typically 10 – 15 minutes regardless of plate type
• Dimensions: 65 x 51 x 35 cm (w x d x h)
• Weight: 60kg
• Electric supply: 110-240V, 50/60Hz

Location: H3 & C2