MJ Research DNA Engine Opticon 2 CFD-3220 Continuous Fluorescence Detector

MJ Research DNA Engine Opticon 2 CFD-3220 Continuous Fluorescence Detector

The MJ Research Opticon is a powerfule Real-time detection tool that
simplifies DNA quantification, genotyping and many other applications.
The BioRad Opticon2 real-time fluorescence detection system features
an innovative optical design and a built-in DNA Engine thermal cycler
for extraordinary real-time performance. The Opticon 2  system
produces reliable, high-resolution results.

The Opticon 2 real-time detector employs an array of 96 blue light-
emitting diodes to excite fluorescent dyes. LEDs provide a long-lived,
maintenance-free excitation source for years of uninterrupted
performance. Emitted fluorescence is then detected by a
photomultiplier tube that not only detects, but also amplifies the
fluorescence signal. A built-in bandpass-filter detection system
separates the specific signal from the background, thereby increasing
the signal-to-noise ratio. This detection strategy delivers
exceptional sensitivity, resulting in the ability to detect as little
as one initial template copy, and to accurately quantify initial
template over a broad dynamic range. This reduces the need for time-
consuming dilutions, even with samples of unknown or widely varying
concentration. The Opticon detector's sensitivity also allows a
reduction in theconsumption of costly reagents without sacrificing
data quality.
 
Specifications:

Fluorescence Excitation Range     470 - 505nm
Fluorescence Detection Range     Ch1: 523 - 543nm; Ch2: 540 - 700nm
Linear Dynamic Range of Starting Copy Number     up to ten orders of
magnitude
Detection Limit of Template Starting Copy Number     Down to single
copy detection
Sample Volume Range     10-100µl (20µl recommended)
Sample Capacity     96-well microplate, or 12 x 0.2ml 8-strip
tubes
Thermal Uniformity     ±0.4°CV within 30 seconds of arrival at 90°C
Thermal Accuracy     Average temp within ±0.3°C of programmed value
at 90°C NIST traceable
Speed of Ramping     Up to 3°C per second
Thermal Range     4° C to 105°C
Gradient Performance     
Temperature Gradient Accuracy     ±0.3°C of target at end columns within
30 seconds of arrival at programmed target, NIST traceable
Thermal Column Uniformity     ±0.4°C, in column, well-to-well,
within 30 seconds of target attainment
Accuracy of Calculated (displayed) Well Temperature     ± 0.4°C of
actual column temperature, NIST traceable
Lowest Temp for Gradient     30°C
Highest Temp for Gradient     105°C
Temperature Differential Range     1.0°C to 24.0°C
Power     100-240 Volt AC, 50-60 Hz
Dimensions     34cm (w) x 47cm (d) x 60cm (h)
Weight     29kg

PDF Manual included

PC and Software not included. Software available to download at
http://opticon-monitor.software.informer.com/3.1/

Unit powers up and appears operative but has not been tested

We believe it is fully operative however as we cannot guarantee this, we are pricing for sale at a spares or repair price

Serial# OP001251