Antigen Retriever 2100

Antigen Retriever 2100

Pre used. with original manual
serial No: OA 24002

The bucket and slide pods are not with it. They can be purchased at Aptum Biologics Ltd

"...the key word about Retriever is reproducibility. Due to fully automatic processing the stainings are always the same, whether they done with a few hours or a few months apart..."

Not only for diagnostics, but also for research (and may well be especially for research pathology) standardization of staining is the key word.

One tool that we have created several years ago, precisely to assure standard staining, is Retriever. In contrast to many other "pressure-cookers" it allows proper recovery of epitopes on formalin-fixed (routine pathology sections) while preserving tissue morphology, shapes of cells, not affecting the shape of the cell's nucleus, and always giving you the same quality and degree of fixed tissue recovery.

History
The idea of Retriever – epitope retrieval by a push of a button – came to me when my PhD student got IHC staining that was clearly different from obtained a month ago. These were very valuable tissues of our transgenic mice, aged, and the availability of the material was limited. Both times the sections were processed in the same microwave under the same conditions, but clearly the degree of processing was entirely different. So I thought how nice it would be if there was a reliable, easy to use piece of equipment where you could process the tissue sections exactly the same way every time.

Technology
Retriever is a “pressure cooker” developed in cooperation with Prestige Medical Ltd, however it is very different from sterilizing equipment. A chip on board the machine controls the profile of heating, pressure and the length of the cycle optimal for most of the routine formalin-fixed, paraffin embedded tissues. Everyone knows that the routine pathology tissues may differ quite substantially by the degree of fixation, by temperature during embedding etc. Over 10 years we were working on the optimization of the parameters for the best unmasking of the antigens in most tissues.

Advantages
First, Retriever requires no special training. And  even a fresh trainee in the lab can use it. Second, it allows simultaneously using several different buffers (often some of your antibodies require citrate buffer, other – acid citrate, and other – EDTA) within one cycle, in one machine. Third, is gives high reproducibility of the results, which allows reviewing together stainings performed with a large time gap in between. Fourth, it is very reliable. Over all the time in the field we had to replace only 2 machines. So getting Retriever you may expect many years of uninterrupted work.

Power 1500W, 240V, 50Hz